Poison is an item used by an Assassin. Damage is total damage done during the duration of the poison. Enemy defense is only applied to the total, not each per second damage indicator.Poison is used by throwing the bottle in an arc and sets off a radius of infliction when it lands 1.5 seconds later. Since this is not a client sided process, expect this to take longer depending on your network latency. The poison is great against stationary enemies, enemies on the other sides of walls and, with skill, taking out entire aggro groups.